Search results for " metabolite"

Postmortem distribution of dihydrocodeine and metabolites in a fatal case of dihydrocodeine intoxication.

1998

A report of a fatal dihydrocodeine ingestion under substitution therapy is given. Quantitation of dihydrocodeine, dihydromorphine, N-nordihydrocodeine, dihydrocodeine-6-, dihydromorphine-6- and dihydromorphine-3-glucuronide was performed simultaneously after solid-phase extraction prior to HPLC analysis, and the analytes were detected using their native fluorescence. Postmortem concentrations of blood samples from different sampling sites as well as from liver, kidney and cerebrum are reported. A hair sample was investigated to prove long-term use of the substitute drug. Site-to-site differences of the analytes from blood samples were very small. The partition behavior of the opioid glucuro…

Composition of mosses, their metabolites and environmental stress impacts

2018

Sūnas ir nozīmīga augu grupa ar plašu izplatību. Sūnu sastāva pētījumi ir kļuvuši aktuāli, ņemot vērā bioloģiski aktīvu savienojumu klātbūtni un augsto izturību pret vides apstākļu mainību. Promocijas darba mērķis ir analizēt sūnu sastāvu, to metabolītus un vides stresa ietekmes uz tiem. Veikta sūnu sekundāro metabolītu ekstrakcijas apstākļu izpēte, kas nodrošina liela skaita vielu identifikāciju. Sūnu lipīdi satur daudzas bioloģiski aktīvas vielas un veikta to aktivitātes izpēte. Sūnu sekundārie metabolīti uzrāda augstu antimikrobiālo aktivitāti un spējas inhibēt vairāku vēža šūnu līniju attīstību un līdz ar to satur perspektīvas vielu grupas izmantošanai biomedicīnā. Vides stresa ietekmes…

Metabolic activation to a mutagen of 3-hydroxy-trans-7,8-dihydroxy-7,8-dihydrobenzo[a]pyrene, a secondary metabolite of benzo[a]pyrene

1987

3-Hydroxy-trans-7,8-dihydroxy-7,8-dihydrobenzo[a]pyrene (3-OH-BP-7,8-diol) was isolated from arylsulfatase/beta-glucuronidase-treated bile of rats to which 3-hydroxybenzo[a]pyrene (3-OH-BP) has been administered. This triol was investigated for mutagenicity in Salmonella typhimurium (reversion to histidine prototrophy of strains TA 97, TA 98, TA 100 and TA 1537) and in V79 Chinese hamster cells (acquisition of resistance to 6-thioguanine). When no exogenous metabolizing system was added the triol was inactive, while 3-OH-BP showed weak mutagenic effects with all four bacterial strains. In the presence of NADPH-fortified postmitochondrial supernatant fraction (S9 mix) of liver homogenate fro…

Metabolites produced by agents of dieback of grapevine in Sicily: Lasiodiplodia mediterranea, Neofusicoccum parvum and N. vitifusiforme

2015

Botryosphaeria dieback was recently reported in West Sicilian vineyards. The disease complex was only associated with isolates of genus Lasiodiplodia in 2007 and together with Diplodia seriata, Neofusicoccum parvum and Neofusicoccum vitifusiforme, this latest reported for the first time on Vitis vinifera in Italy. Moreover, a representative isolate of each species was inoculated on green shoots and fulfilled Koch’s postulates, although the lengths of vascular discolourations were variable depending on the species. As well as the other GTD causal agents, the expression of foliar symptoms (pale colour along the margins and between veins) was also occasional in Sicily, it was hypothesized that…

Content variability of bioactive secondary metabolites in Hypericum perforatum L

2021

Abstract St John’s Wort (Hypericum perforatum L.; Hypericaceae) is a perennial medicinal herb widespread and largely used in folk medicine inside the Mediterranean basin. Many bioactive compounds have been identified within its extracts. Under a pharmacological point of view, the most important of them belong to the chemical classes of naphthodianthrones, phloroglucinols and polyphenols. Many factors have been claimed responsible for the phytochemical variability in Hypericum perforatum, such as genotype, geographical origin, harvesting stage and age of the plants. Yet, when harvested plant material is addressed to the industry, the standardization of the active ingredients over cultivation…

Interspecific variation in total phenolic content in temperate brown algae

2017

Marine algae synthesize secondary metabolites such as polyphenols that function as defense and protection mechanisms. Among brown algae, Fucales and Dictyotales (Phaeophyceae) contain the highest levels of phenolic compounds, mainly phlorotannins, that play multiple roles. Four temperate brown algae (Cystoseira amentacea, Cystoseira compressa, Dictyopteris polypodioides and Padina pavonica) were studied for total phenolic contents. Total phenolic content was determined colorimetrically with the Folin-Ciocalteu reagent. Significant differences in total phenolic content were observed between leathery and sheetlike algae and also within each morphological group. Among the four species, the she…

Antiproliferative activity against leukemia cells of sesquiterpene lactones from the Turkish endemic plant Centaurea drabifolia subsp. detonsa

2017

The apolar organic extract obtained from aerial parts of Centaurea drabifolia Sibth. & Sm. subsp. detonsa (Bornm.) Wagenitz, growing wild in Turkey, was investigated for the first time for its secondary metabolite composition. Seven sesquiterpene lactones belonging to the guaiane class (1-7), including the new compound 4, along with a fatty acid lactone derivative (8), were isolated. The structures of these compounds were established by spectroscopic analysis, including 2D NMR spectroscopic techniques, with the stereostructure of the new guaiane 4 determined with the help of MTPA derivatization. Cytotoxic activities of compounds 1-7 were evaluated against two cancer cell lines, namely acute…

2021

Drosera rotundifolia L. is a carnivorous plant used in traditional medicine for its therapeutic properties. Because of its small size, its collection in nature is laborious and different cultivation methods have been studied to ensure availability. However, only a few studies exist where the lab-grown sundew tissue and field-grown sundew would have been compared in their functionality or metabolic profiles. In this study, the antioxidant and antiviral activities of lab-grown and field-grown sundew extracts and their metabolic profiles are examined. The effect of drying methods on the chromatographic profile of the extracts is also shown. Antioxidant activity was significantly higher (5–6 ti…

Metabolic profiling of ripe olive fruit in response to moderate water stress

2013

a b s t r a c t The concentrations of different metabolites in olive (Olea europaea (L.)) fruit at harvest can be affected by water availability, with significant consequences on the composition and the quality of the resulting oil. The aim of the present study was to profile the metabolic composition of ripe olives (cv. Cipressino) grown under water-stress and irrigated conditions applied during the last part of the fruit developmental cycle (from pit hardening to commercial harvest). The imposed conditions resulted in a moderate water stress (−3.5 MPa) at the end of the experimental period. Samples (pulp + skin) of fruit collected at the stage of complete epicarp pigmentation were analyze…

A systematic review and combined analysis of therapeutic drug monitoring studies for longacting risperidone

2017

Introduction: This systematic review of therapeutic drug monitoring (TDM) identifies three long-acting injectable (LAI) risperidone formulations. Areas covered: Limited data is available on two formulations (RBP-7000 and in Situ Microparticle), but 20 TDM articles on the microsphere formulation were found. Risperidone TDM includes the serum concentrations of risperidone and its active metabolite, 9-hydroxyrisperidone, used for calculating: 1) the risperidone/9-hydroxyrisperidone (R/9-OH-R) ratio (a measure of CYP2D6; values >1 are indicative of a CYP2D6 poor metabolizer) and 2) the total risperidone concentration-to-dose (C/D) ratio (a measure of risperidone clearance with a normal value…